Inorganic / Biological / Materials
Our research in bioinorganic chemistry focuses on biologically relevant metals that are very sensitive to hydrolysis – mainly iron and titanium – and addresses how biology handles these difficult metals. Problems relate to metal uptake and transport, potential therapeutic applications thereof, and biomineralization. Some of our work includes:
Biologically and Environmentally Relevant Titanium Coordination Chemistry:
Doing Ti(IV) chemistry in water, in the presence of oxygen and at neutral pH is tough, because Ti(IV) hydrolyzes so easily. We have worked to characterize complexes using ligands relevant to the coordination of Ti(IV) in biology and/or the environment that provide important models and allow us to address the questions below.
Titanium and Proteins:
Titanium(IV) is a bioactive metal ion, and Ti(IV)-containing molecules have been promising as anticancer drugs. We all have more titanium in our bodies than most people realize, and that is before getting a titanium alloy orthopedic implant, ingesting TiO2 in a pharmaceutical formulation, etc. We are characterizing the interactions between titanium ions and biomolecules to explore the nature of this bioactivity, and to exploit it for human benefit.
Usually we in the Valentine Group try to prevent titanium minerals from forming, but one of our projects asks whether organisms might use titanium in mineral form, or perhaps even actively biomineralize titanium. We have looked at whole organisms and worked with biomolecules modeling those found in diatoms, to understand the controlled mineraliztion of titanium.
Iron management proteins in higher organisms include transferrin. Serum transferrin in vertebrates is an approx. 80 kD bilobal protein that came from an ancient gene duplication of a primitive monolobal form. A monolobal transferrin (called nicatransferrin or nicaTf) was discovered in a group of marine invertebrate chordates called ascidians (or sea squirts). (Sea squirts are also well known to inorganic chemists for the ability of
some species to sequester remarkable concentrations of unusual metals like vanadium and titanium.) We isolated the protein from the native organism and also expressed it in yeast. We are working to characterize this protein, partly to understand better how nature evolved the capacity to manage very hydrolysis-prone metals.